LOCUS NC_008598 55939 bp DNA circular BCT 18-APR-2007 DEFINITION Bacillus thuringiensis str. Al Hakam plasmid pALH1, complete sequence. ACCESSION NC_008598 VERSION NC_008598.1 GI:118445179 KEYWORDS . SOURCE Bacillus thuringiensis str. Al Hakam ORGANISM Bacillus thuringiensis str. Al Hakam Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group. REFERENCE 1 (bases 1 to 55939) AUTHORS Challacombe,J.F., Altherr,M.R., Xie,G., Bhotika,S.S., Brown,N., Bruce,D., Campbell,C.S., Campbell,M.L., Chen,J., Chertkov,O., Cleland,C., Dimitrijevic,M., Doggett,N.A., Fawcett,J.J., Glavina,T., Goodwin,L.A., Green,L.D., Han,C.S., Hill,K.K., Hitchcock,P., Jackson,P.J., Keim,P., Kewalramani,A.R., Longmire,J., Lucas,S., Malfatti,S., Martinez,D., McMurry,K., Meincke,L.J., Misra,M., Moseman,B.L., Mundt,M., Munk,A.C., Okinaka,R.T., Parson-Quintana,B., Reilly,L.P., Richardson,P., Robinson,D.L., Saunders,E., Tapia,R., Tesmer,J.G., Thayer,N., Thompson,L.S., Tice,H., Ticknor,L.O., Wills,P.L., Gilna,P. and Brettin,T.S. TITLE The Complete Genome Sequence of Bacillus thuringiensis Al Hakam JOURNAL J. Bacteriol. 189, 3680-3681 (2007) PUBMED 17337577 REFERENCE 2 (bases 1 to 55939) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (29-NOV-2006) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 55939) AUTHORS Lucas,S., Pitluck,S., Chertkov,O., Goodwin,L.A., Han,l., Munk,A.C., Saunders,E., Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P., Martinez,A.D., Misra,M., Tapia,R., Thayer,N.N., Xie,G., Brown,N., Green,L.D., Hill,K.K., Jackson,P.J., Longmire,J., Rubin,E. and Richardson,P. TITLE Direct Submission JOURNAL Submitted (14-NOV-2006) Joint Genome Institute, Department of Energy, 2800 Mitchell Drive, Walnut Creek, Ca 94598, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CP000486. Bacillus thuringiensis Al Hakam was collected at a suspected bioweapons facility in Iraq by the United Nations Special Commission (Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Radnedge, L, Agron, PG Hill, KK, Jackson, PJ, Ticknor, LO, Keim, P, and Andersen, GL. Appl Environ Microbiol 2003. 69:2755-64). Plasmid and fosmid libraries were prepared at the Joint Genome Institute in Los Alamos (JGI-LANL), NM. Shotgun sequencing was performed at the JGI Production Genomics Facility (JGI-PGF) in Walnut Creek, CA. Draft assemblies were based on 246217 total reads. All libraries provided 23x coverage of the genome. The Phred/Phrap/Consed software package (www.phrap.com) was used for sequence assembly and quality assessment. After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected manually or by transposon bombing (Epicentre Biotechnologies) of bridging clones. Gaps between contigs were closed by editing in Consed, custom primer walk or PCR amplification. A total of 25321 additional reactions were necessary to close gaps and to raise the quality of the finished sequence. The completed genome sequence of B. thuringiensis Al Hakam achieves an average of 24-fold sequence coverage per base with error rate less than 1 in 100,000. Gene predictions were obtained using Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis of the gene predictions was performed by comparing coding sequences against the PFam, BLOCKS and Prodom databases. Gene definitions and functional classes were added manually by a team of annotators at JGI-LANL, using BLAST results in addition to information from the basic analysis. COMPLETENESS: full length.
Материалы сообщества доступны в соответствии с условиями лицензии CC-BY-SA , если не указано иное.